July 18, 2017 The amino acid positions of titin variants had been identified with the use of the UniProt titin sequence , and mutations were reported with the use of Human Genome Variation Culture nomenclature . Variant confirmation and genotyping were performed by means of one or even more of the methods: polymerase-chain-reaction amplification followed by dideoxy sequencing; restriction digestion and gel electrophoresis26; and RNA sequencing of cardiac cells.33 Statistical Analysis Association, cross-cohort, and cross-group analyses were performed with the use of Fisher’s exact check, exact conditional testing of independence, or goodness-of-fit tests, unless specified otherwise.Abdominal pregnancies constitute about one in 10,000 deliveries. In this rare instance, caregivers 40 years back believed the fetus experienced aborted, since the mother’s tummy stopped growing. They were wrong; the infant stayed in the pelvic region, where he/she considered stone. The stone fetus is called a lithopedion. Litho – is the Greek prefix meaning rock, and -pedion may be the Greek suffix meaning kid. The fetus was estimated to weigh around 4 pounds. The fetus resembled a baby statue. Find photos of stone fetuses here. Immune system naturally protects the body from foreign objects, calcifies the fetus dies themAfter, the mother’s immune system goes to work protecting her personal body from the fetus that’s deemed by the body to become a international object.